INDUKSI METABOLIT PORPHYROMONAS GINGIVALIS PADA PRODUKSI RADIKAL SUPEROKSID NETROFIL

INDUCTION OF PORPHYROMONAS GINGIVALIS METABOLITES ON NEUTROPHIL SUPEROXIDE RADICAL PRODUCTION

Abstract

Radical suproxide is a kind of reactive oxygen species (ROS) which causes oxidative damage by attacking biomolecules
such as protein, lipid, DNA, etc. Over production of radical superoxide becomes a centre of the generation of other radical
species and oxidants, and responsible for occurring many pathological processes. The main source of radical superoxide is
to activate neutrophil. Therefore, it was speculated that induction of neutrophil by periodontitis bacteria Porphyromonas
gingival is potential to induce superoxide production, only a few study, however concerned with this topic. This study
aimed to know the pathogenicity of P. gingivalis by demonstrating the potency of P. gingivalis metabolites to induce
production of radical superoxide by neutrophil. This study was conducted experimentally in vitro using posttest only
design. Superoxide radical production was demostrated by means of Nitrobluetetrazolium (NBT) slide assay and
spectro photometric. The results showed that P. gingivalis metabolites induced neutrophil to produce a large amount of
radical superoxide both intra and extra cellular. Its radical superoxide production affected degranulation, apoptosis, and
lysis of neutrophil. In conclusion, neutrophil responses against P. gingivalis generated prooxidative and toxic condition.
This mechanism may explain the pathogenicity of P. gingivalis on many pathological processes.