EFFECT OF CASSAVA TUBER PROCESSING ON Candida albicans GROWTH ON MANIHOT DEXTROSE AGAR
DOI:
https://doi.org/10.32734/idjpcr.v8i01.20763Keywords:
Candida albicans, Fungi\, Media, CassavaAbstract
This study evaluated the effect of cassava tuber processing on Candida albicans growth on Manihot Dextrose Agar (MDA). The sliced cassava tubers were divided into three groups. G1 was milled and dried in an oven at 60°C. G2 was directly dried in the oven. G3 was milled without drying. Each group was soaked in distilled water (1,000 mL) for 15 min and filtered with a flannel cloth. The filtrate was oven-dried, then pulverized to obtain the cassava powder. The powder (8 g) was combined with dextrose (20 g) and agar (15 g). The mixture was suspended in distilled water (1,000 mL), boiled, and sterilized at 121°C for 15 min. The solution was placed into a petri dish and allowed to form agar media. The media derived from G1, G2, and G3 were considered as F1, F2, and F3. A suspension of C. albicans was cultured on the media and incubated for 48 h. The fungal growth was observed by calculating the viable colonies. The data were analyzed with one-way ANOVA and followed by Duncan’s test at the confidence level of 95%. The results showed that C. albicans grown on media F1, F2, and F3 had colony numbers of 128±2.08 x 105 cfu/mL, 64±5.57 x 105 cfu/mL, and 44±3.51 x 105 cfu/mL, respectively. Statistical analysis indicated a significant difference in the fungal growth on the three media (p < 0.05). This study proved that the processing methods of cassava tubers for MDA significantly affected the growth of C. albicans.
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. Idris S, Rosnah S, Nor M, Mokhtar M, Abdul Gani S. Physicochemical composition of different parts of cassava (Manihot esculenta Crantz) plant. Food Res. 2020;4(S1):78–84. DOI: http://dx.doi.org/10.26656/fr.2017.4(S1).S33.
. Chemeda A, Bussa N. Nutritional and anti-nutrient content of cassava (Manihot esculenta Crantz) grown in Hararghe, Eastern Ethiopia: as affected by varieties and processing methods. Glob Sci J. 2018;6(4):160–167. DOI: http://dx.doi.org/10.5829/idosi.ajn.2019.34.46.
. Morgan N, Chot. Cassava: Nutrient composition and nutritive value in poultry diets. Anim Nutr. 2016;2(4):253-261. DOI: https://doi.org/10.1016/j.aninu.2016.08.010.
. Kim DS, Iida F. Nutritional composition of cassava (Manihot esculenta) and its application to elder-friendly food based on enzyme treatment. Int J Food Prop. 2023;26(1):1311–1323. DOI: http://dx.doi.org/10.1080/10942912.2023.2213410.
. Walker G, White N. Introduction to fungal physiology. In: Fungi: biology and applications. 3rd ed. New Jersey: John Wiley & Sons, Inc.; 2018.
. Wongjiratthiti A, Yottakot S. Utilization of local crops as alternative media for fungal growth. Pertanika J Trop Agric Sci. 2017;40(2):295–304.
. Shinde S, Kanase S. Cassava starch as a partial substitute for agar-agar in microbiological media. Bull Environ Pharmacol Life Sci. 2023;1:213 215.
. Ravimannan N, Arulanantham S, Pathmanathan, Kularajani N. Alternative culture media for fungal growth using different formulations of protein sources. Ann Biol Res. 2014;5(1):36–39.
. Borah A, Mahanta J, Narzary Z. Formulation of cost-effective alternative fungal culture media using local resources. J Adv Plant Sci. 2020;10(2):31–36.
. Mundin S, Kluczkovki AM, Rodrigues JC, Brito VH, Fernandes OC. Cyanide influence on the growth of mycotoxigenic fungi from cassava flour in vitro. Afr J Microbiol Res. 2015;9(17):1184–1188. DOI: http://dx.doi.org/10.5897/AJMR2015.7433.
. Patilaya P, Firmansyah A. Metode pembuatan ekstrak kering umbi singkong sebagai media pertumbuhan jamur. Medan 2019; IDS000002761.
. Rashmi H, Devantha C. Review on solid-liquid separation using various conditioning methods. IOP Conf Ser Mater Sci Eng. 2021; 1166(1):012002. DOI: http://dx.doi.org/10.1088/1757-899X/1166/1/012002.
. Parambath S, Dao A, Kim HY, Zawahir S, Izquierdo AA, Tacconelli E, et al. Candida albicans - a systematic review to inform the World Health Organization fungal priority pathogens list. Med Mycol. 2024;62(6):myae074. DOI: http://dx.doi.org/10.1093/mmy/myae045.
. Moya-Salazar J, Rojas R. Comparative study for identification of Candida albicans with germ tube test in human serum and plasma. Clin Microbiol Infect Dis. 2018;3(3):1–4. DOI: http://dx.doi.org/10.15761/CMID.1000143.
. Putri SK, Wahyuni S, Fajarna F. The use of cassava as an alternative medium for Candida albicans culture. J SAGO. 2022;4(1):44–51. DOI: http://dx.doi.org/10.30867/gikes.v4i1.1052.
. Khusnul, Nafisa G, Hidana R, Virgianti DP. Influence of the growth of Candida albicans on several alternative media. Adv Health Sci Res. 2020;26:5 8. DOI: https://doi.org/10.2991/ahsr.k.200523.002.
. Agbara GI, Babagana B. Effects of differently treated cassava flours blended with bambara groundnut flour on the bread-making potential of two Nigerian wheat cultivars. Arid Zone J Eng Technol Environ. 2022;18(4):555-574.
. Bayata A. Review on nutritional value of cassava for use as a staple food. Sci J Anal Chem. 2019;83-91;7(4). DOI: http://dx.doi.org/10.11648/j.sjac.20190704.12.
. Ismaila AR, Alakali JS, Atume TG. Effect of local processing techniques on the nutrients and anti-nutrient content of bitter cassava (Manihot esculenta Crantz). Am J Food Sci Technol. 2018;6(3):92-97. DOI: http://dx.doi.org/10.12691/ajfst-6-3-1.
. Peña A, Sánchez NS, González-López O, Calahorra M. Mechanisms involved in the inhibition of glycolysis by cyanide and antimycin A in Candida albicans and its reversal by hydrogen peroxide. A common feature in Candida species. FEMS Yeast Res. 2015;15(8):fov083. DOI: http://dx.doi.org/10.1093/femsyr/fov083.
. Okonkwo UC, Onokwai AO, Okeke CL, Osueke CO, Ezugwu CA, Diarah RS, Aremu CO. Investigation of the effect of temperature on the rate of drying moisture and cyanide contents of cassava chips using oven drying process. Int J Mech Eng Techol. 2019;10(1):1507-1520.
. Onyango S, Abong G, Okoth M, Kilalo D, Mwang’ombe A. Effect of pre-treatment and processing on nutritional composition of cassava roots, millet, and cowpea leaves flours. Front Sustain Food Syst. 2021;5:625735. DOI: http://dx.doi.org/10.3389/fsufs.2021.625735.
. Chisenga SM, Workneh TS, Bultosa G, Laing M. Proximate composition, cyanide contents, and particle size distribution of cassava flour from cassava varieties in Zambia. AIMS Agric Food. 2019;4(4):869-891. DOI: https://doi.org/10.3934/agrfood.2019.4.869.
. Scaria SS, Balasubramanian B, Meyyazhagan A, Gangwar J, Jaison JP, Kurian JT, Pushparaj K, Pappuswamy M, Park S, Joseph KS. Cassava (Manihot esculenta Crantz) - a potential source of phytochemicals, food, and nutrition - an updated review. eFood. 2024;5(1):e127. DOI: https://doi.org/10.1002/efd2.127.
. Ndubuisi ND, Chidiebere ACU. Cyanide in cassava: a review. Int J Genomics Data Min. 2018;3(1):1–10. DOI: http://dx.doi.org/10.29011/2577-0616.000118.
. Viji V, Veena S, Karthikeyan S, Jeeva M. Cassava-based substrates - conducive media for mass multiplication of Trichoderma asperellum. J Root Crop. 2018;44(1):41–46.
. Nurdin E, Nurdin GM. Perbandingan variasi media alternatif dengan berbagai sumber karbohidrat terhadap pertumbuhan Candida albicans. Bionature. 2020;21(1):1–5. DOI: http://dx.doi.org/10.35580/bionature.v21i1.13920.
. Safitri AN, Qurrohman MT. Perbandingan pertumbuhan jamur candida albicans pada media alami jagung, singkong dan ubi jalar kuning. J Indones Med Lab Sci. 2022;3(2):97–107. DOI: http://dx.doi.org/10.53699/joimedlabs.v3i2.76.
. Chisenga SM, Workneh TS, Bultosa G, Laing M. Proximate composition, cyanide contents, and particle size distribution of cassava flour from cassava varieties in Zambia. AIMS Agric Food. 2019;4(4):869–891. DOI: http://dx.doi.org/10.3934/agrfood.2019.4.869.
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