Rhizosphere microbial resistance test to fungicide Dimetomorph in vitro

Putri Megawati Sari; Hanik Faizah; Irul Hidayati; Cici Fitri Yani

doi:10.32734/jopt.v13i2.21669

Authors

Putri Megawati Sari UIN Sunan Ampel Surabaya
Hanik Faizah UIN Sunan Ampel Surabaya
Irul Hidayati UIN Sunan Ampel Surabaya
Cici Fitri Yani UIN Sunan Ampel Surabaya

DOI:

https://doi.org/10.32734/jopt.v13i2.21669

Keywords:

compatibility, dimetomorph fungicide, pesticide, resistance, rhizosphere microbial, sensitivity

Abstract

Dimethomorph is a systemic fungicide utilized within Indonesian agricultural sector. Inappropriate application of dimethomorph fungicide may result in environmental contamination and the development of resistance among rhizosphere microorganisms. This study aims to ascertain the responses of rhizosphere microbial isolates to dimethomorph fungicide, thereby providing critical insights for evaluating the efficacy of this fungicide against rhizosphere microbes. Such information is essential for formulating plant pest and disease management strategies that utilize more suitable and sustainable dosages. The investigation involved testing bacterial isolates MR-B01, MR-B02, MR-B03, MR-B04, and fungal isolate MR-F01 against dimethomorph fungicide employing the agar diffusion method with disc paper. The experimental design incorporated a treatment factor with five variation concentrations of dimethomorph fungicide (0 g/L, 0.5 g/L, 1 g/L, 1.5 g/L, and 2 g/L), executed with ten repetitions. The findings indicated that varying concentrations of dimethomorph fungicide exhibited distinct effects on the resistance of the tested microbial isolates, specifically bacteria MR-B01, MR-B02, MR-B03, MR-B04, and fungal isolate MR-F01.

Downloads

Download data is not yet available.

References

Ajith, C. R., Pankaja, N. S., Supriya, S., Mahadeva, J., & Umashankar Kumar, N. (2024). Compatibility Assessment of Native Non-Rhizospheric Trichoderma Isolates with Various Fungicides. Journal of Advances in Biology & Biotechnology, 27(9), 988-999.

Aleksandrova, Y. I., Shurpik, D. N., Nazmutdinova, V. A., Mostovaya, O. A., Subakaeva, E. V., Sokolova, E. A., & Stoikov, I. I. (2023). Toward Pathogenic Biofilm Suppressors: Synthesis of Amino Derivatives of Pillar [5] Arene and Supramolecular Assembly with DNA. Pharmaceutics, 15(2), 476.

Amin, M., Akrami, S., Haghparasty, F., & Hakimi, A. (2023). In vitro Antibacterial Activities of Essential Oils and Extracts of Six Herbals Against Gram Positive and Gram Negative Bacteria. Toxicology and Environmental Health Sciences, 15(1), 53-60.

Archana, S., Hubballi, M., Ranjitham, T. P., Prabakar, K., & Raguchander, T. (2012). Compatibility of Azoxystrobin 23 SC with Biocontrol Agents and Insecticides. Madras Agricultural Journal, 99(4/6), 374-377.

Barnett, H. L, & Hunter, B. B. (1998). Illustrated Genera of Imperfect Fungi. USA: Prentice-Hall, Inc.

Bergey, D. H. (1994). Bergey's manual of Determinative Bacteriology. Lippincott Williams & Wilkins.

Darsono, P. V., & Fajriannor, M. (2020). Aktivitas Antibakteri Ekstrak Dadangkak (Hydrolea spinosa) terhadap Bakteri Bacillus subtilis, Staphylococcus aureus dan Escherichia coli. Jurnal Ilmiah Ibnu Sina, 5(1), 117-127.

Davis, W. W., & Stout, T. R. (1971). Disc Plate Method of Microbiological Antibiotic Assay: I. Factors Influencing Variability and Error. Applied Microbiology, 22(4), 659-665.

Dotulong, G., Umboh, S., & Pelealu, J. (2019). Uji Toksisitas Beberapa Fungisida Nabati terhadap Penyakit Layu Fusarium (Fusarium oxysporum) pada Tanaman Kentang (Solanum tuberosum L.) secara in vitro. Jurnal Bios Logos, 9(2), 91-101.

Erfin, N., Sandiah, L., Malesi. (2016). Identifikasi Bakteri Azosprillium dan Azotobacter pada Rhizosfer Asal Komba-komba (Chromolaena odorata). Jitro, 3(2), 31-38.

European Food Safety Authority (EFSA), Alvarez, F., Arena, M., Auteri, D., Binaglia, M., Castoldi, A. F., & Villamar‐Bouza, L. (2023). Peer Review of the Pesticide Risk Assessment of the Active Substance Dimethomorph. EFSA Journal, 21(6), 1-37.

Escudero-Leyva, E., Alfaro-Vargas, P., Muñoz Arrieta, R., Charpentier-Alfaro, C., Granados Montero, M. D. M., Valverde Madrigal, K. S., & Mora Villalobos, J. A. (2022). Tolerance and Biological Removal of Fungicides by Trichoderma Species Isolated from the Endosphere of Wild Rubiaceae Plants. Frontiers in Agronomy, 3, 772170.

FAO. (2023). Statistical Yearbook World Food and Agriculture. Roma. https://doi.org/10.4060/cc8166en.

Fauziah, D. N. (2023). Pengaruh Fungisida Asam Fosfit, Dimetomorf, dan Metalaksil Terhadap Perkecambahan Konidia Peronosclerospora maydis dan Intensitas Penyakit Bulai pada Tanaman Jagung. Skripsi. Bandar Lampung: Fakultas Pertanian. Universitas Lampung.

Gandjar, I. (2006). Mikologi Dasar dan Terapan. Yayasan Obor Indonesia.

Ginting, M., Manalu, K., & Nasution, R. A. (2024). Population and Characterization of Rhyzospheric Bacteria of Pineapple Plant (Ananas comosus L. Merr) on The Highland Land of Lumban Sihite Village, Regency Dairi. Jurnal Biologi Tropis, 24(2), 535-540.

Hajijah, H., Mariana, M., & Pramudi, M. I. (2022). Uji Resistensi Colletotrichum sp. Asal Cabai Hiyung terhadap Fungisida Berbahan Aktif Klorotalonil dan Mankozeb. Jurnal Proteksi Tanaman Tropika, 5(2), 455-465.

Hasibuan, A., Nasution, S. P., Yani, F. A., Hasibuan, H. A., & Firzah, N. (2022). Strategi Peningkatan Usaha Tani Padi Sawah untuk Meningkatkan Perekonomian Masyarakat Desa. ABDIKAN: Jurnal Pengabdian Masyarakat Bidang Sains dan Teknologi, 1(4), 477-490.

Helmiyati, A. F. (2010). Pengaruh Konsentrasi Tawas terhadap Pertumbuhan Bakteri Gram Positif dan Negatif. Jurnal Pangan dan Gizi, 1(1).

Hindersah, R., Subardja, V. O., Suryatmana, P., & Sudirja, R. (2024). Properties of Azotobacter as Biofertilizier and Biocontrol of Fusarium. Agrologia, 13(1), 17-23.

Holderman, J. L., Thomas IV, J. C., Schlanger, K., Black, J. M., Town, K., St. Cyr, S. B., & Kirkcaldy, R. D. (2021). Sustained Transmission of Neisseria Gonorrhoeae with High Level Resistance to Azithromycin, in Indianapolis, Indiana, 2017–2018. Clinical Infectious Diseases, 73(5), 808-815.

Khulillah, I. N., Abadi, A. L., & Aini, L. Q. (2019). Pengaruh Fungisida terhadap Keanekaragaman Bakteri Tanah di Kota Batu. Jurnal Tanah dan Sumberdaya Lahan, 6(2), 1209-1218.

Kubicek, C. P., Steindorff, A. S., Chenthamara, K., Manganiello, G., Henrissat, B., Zhang, J., & Druzhinina, I. S. (2019). Evolution and Comparative Genomics of The Most Common Trichoderma Species. BMC Genomics, 20, 1-24.

Madigan, M. T., Martinko, J. M., & Parker, J. (1997). Brock Biology of Microorganisms (Vol. 11). Upper Saddle River, NJ: Prentice hall.

Magvirah, T., Marwati, M., & Ardhani, F. (2020). Uji Daya Hambat Bakteri Staphylococcus aureus menggunakan Ekstrak Daun Tahongai (Kleinhovia hospita L.). Jurnal Peternakan Lingkungan Tropis, 2(2), 41-50.

Nikaido, H. (2003). Molecular Basis of Bacterial Outer Membrane Permeability Revisited. Microbiology and Molecular Biology Reviews, 67(4), 593-656.

Ni'mah, F., & Yuliani, Y. (2022). Pengaruh Azospirillum sp. dan Biochar Tongkol Jagung terhadap Pertumbuhan Glycine max L. pada Tanah Salin. LenteraBio:Berkala Ilmiah Biologi, 11(3), 385-394.

Novianty, R., Dahliaty, A., Nasution, N. I., & Haryati, H. (2020). Biodegradation of used Baby Diapers using Cellulolitic Fungus and Bacteria with Solid Fermentation. Jurnal Inovasi Pendidikan dan Sains, 1(1), 13-19.

Nuraini, C., Saida, S., Suryanti, S., & Nontji, M. (2020). Isolasi dan Identifikasi Bakteri Rhizosfer Tanaman Jagung pada Fase Vegetatif dan Generatif. AGrotekMAS Jurnal Indonesia: Jurnal Ilmu Peranian, 1(1), 24 30.

Putri, A. L., & Kusdiyantini, E. (2018). Isolasi dan Identifikasi Bakteri Asam Laktat dari Pangan Fermentasi Berbasis Ikan (Inasua) yang diperjualbelikan di Maluku-Indonesia. Jurnal Biologi Tropika, 1(2), 6 12.

Raissa, D. (2017). Pengaruh Aplikasi Beberapa Jenis Fungisida Melalui Teknik Perlakuan Benih terhadap Insiden Penyakit Bulai Jagung (Peronosclerospora spp.). Skripsi. Bandar Lampung: Fakultas Pertanian. Universitas Lampung.

Ristiari, N. P. N., Julyasih, K. S. M., & Suryanti, I. A. P. (2019). Isolasi dan Identifikasi Jamur Mikroskopis pada Rizosfer Tanaman Jeruk Siam (Citrus Nobilis Lour.) di Kecamatan Kintamani, Bali. Jurnal Pendidikan Biologi Undiksha, 6(1), 10-19.

Rosmania, R., & Yanti, F. (2020). Perhitungan Jumlah Bakteri di Laboratorium Mikrobiologi Menggunakan Pengembangan Metode Spektrofotometri. Jurnal Penelitian Sains, 22(2), 7686.

Ruiz Tello, K. S. (2023). Sensibilidad En El Desarrollo de Bacillus Subtilis F. Cohn Al Efecto de Diez Plaguicidas de Síntesis Química En Condiciones Controladas de Laboratorio.

Saha, S., Pharate, S., Thosar, R. U., & Chavan, V. (2023). Compatibility of Trichoderma asperelloides with Fungicides Controlling Downy Mildew and Powdery Mildew in Grapes. Grape Insight, 32-36.

Sanam, T., Triveni, S., Satyanaryana, J., Nerella, S. G., Chari, K. D., & Desai, S. (2021). Evaluation of Diverse Range Microbes for Their Plant Growth Promoting Abilities and Their Pesticide Compatibility. Environment Conservation Journal, 22(3), 47-58.

Sangkala., Bakhtiar, A., & Syam’un, E. (2021). Keragaman Morfologi Bakteri Penambat Nitrogen dan Pelarut Fosfat dari Berbagai Lingkungan Agroekosistem di Kabupaten Takalar. Jurnal Biotek, 9(1), 93-112.

Sanuddin, M., Hadriyati, A., & Sari, I. P. (2022). Uji Aktivitas Antibakteri Staphylococcus aureus dan Escherichia coli terhadap Senyawa Sintesis Difeniltin (IV) Metil Ditiokarbamat. Lumbung Farmasi Jurnal Ilmu Kefarmasian, 3(1), 46-50.

Saraswati, R., Prihatini, T., & Hastuti, R. D. (2004). Teknologi Pupuk Mikroba untuk Meningkatkan Efisiensi Pemupukan dan Keberlanjutan Sistem Produksi Padi Sawah. Tanah Sawah dan Teknologi Pengelolaannya. Pusat Penelitian dan Pengembangan Tanah dan Agroklimat. Bogor, 169-189.

Sari, E. M., Suwirmen., & Noli, Z. A. (2014). Pengaruh Penggunaan Fungisida (Dithane M 45) terhadap Pertumbuhan Tanaman Jagung (Zea mays L.) dan Kepadatan Spora Fungi Mikoriza Arbuskula (FMA). Jurnal Biologi Unand, 3(3), 188-194.

Sumardiyono, C. (2008). Ketahanan Jamur terhadap Fungisida di Indonesia. Jurnal Perlindungan Tanaman Indonesia, 14(1), 1-5.

Syaifullah, Y., & Wati, F. T. (2023). Analisis Pencemaran Tanah di Desa Gembol Penjawaran Banjarnegara. MASALIQ, 3(5), 935-940.

Tkach, Y., Bunas, A., Starodub, V., Pylypchuk, T., & Gumeniuk, I. (2021). The Effect of Chemicals of Plant Protection Products on Soil Microbiocenoses.

Ula, A., & Mizani, Z. M. (2022). Pemanfaatan Limbah Kulit Bawang Putih menjadi Biopestisida Alami pada Kelompok Tani di Desa Klorogan, Kecamatan Geger, Kabupaten Madiun. Jurnal Tadris IPA Indonesia, 2(1), 111-120.

Wang, P. F., Qiu, H. Y., Ma, J. T., Yan, X. Q., Gong, H. B., Wang, Z. C., & Zhu, H. L. (2015). Dihydropyrazoles Containing Morpholine: Design, Synthesis and Bioassay Testing as Potent Antimicrobial Agents. RSC advances, 5(32), 24997-25005.

Wendersteyt, N. V., Wewengkang, D. S., & Abdullah, S. S. (2021). Uji Aktivitas Antimikroba dari Ekstrak dan Fraksi Ascidian Herdmania Momus dari Perairan Pulau Bangka Likupang terhadap Pertumbuhan Mikroba Staphylococcus aureus, Salmonella typhimurium dan Candida albicans. Pharmacon, 10(1), 706-712.

Walpole, R.E. (1995). Pengantar Statistika Edisi 3. PT. Gramedia Pustaka Utama, Jakarta.

Wulandari, F. (2020). Aplikasi Beberapa Fungisida Sebagai Perlakuan Benih terhadap Penyakit Downy Mildew (Peronosclerospora maydis) pada Tanaman Jagung. Tugas Akhir. Lampung: Fakultas Budidaya Tanaman Pangan. Politeknik Negeri Lampung.

Wulandari, W. (2025). Identifikasi Bakteri Plant Growth Promoting Rhizobacteria pada Rhizosfer Tanaman Kelapa Sawit. (Doctoral dissertation, Institut Pertanian Stiper Yogyakarta).

Zhang, H., Song, J., Zhang, Z., Zhang, Q., Chen, S., Mei, J., & Fang, H. (2021). Exposure to Fungicide Difenoconazole Reduces the Soil Bacterial Community Diversity and the Co Occurrence NetworkComplexity. Journal of Hazardous Materials, 405, 124208.